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Experimental design, materials and methods
Acknowledgments This work was financially supported by the Modern Agricultural Industry Technology System (CARS-37), the Key Project of Agricultural Fine Breeding of Shandong Province (2012LZ15), and the Support Program of Tai Mountain Scholar Talent Team for Agricultural Breeding (2014LZ07-04).
Data The data presented in this article is a pairwise comparison of the MU activities of the traditional β-galactosidase assay and a single-step automated method [2]. 4 different strains with MU activities (0–10, 40–70, 500–900, and 1800–2400 respectively) were assayed using both methods.
Experimental design, materials and methods
Specifications Table
Value of the data
Data Tables 1 and 2 describe the HLA class I and II data related to susceptibility/resistance to pemphigus foliaceus and pemphigus vulgaris in reviewed Brazilian reports. Tables 3–8 show the HLA class I (-A, -B, -C) and class II (-DRB1, -DQA1, -DQB1) profile performed in pemphigus foliaceus and pemphigus vulgaris patients from Southeastern Brazil.
Experimental design, materials, and methods A summary of Brazilian data regarding associations between HLA and pemphigus was obtained in PubMed. A hundred and sixty-nine patients followed up at the University Hospital of the Ribeirão Preto Medical School of the University of São Paulo, Brazil, were evaluated. Eighty-six and 83 patients exhibited PF and PV, respectively. The control group consisted of 1592 healthy individuals living in the northeastern region of the state of São Paulo, Southeastern Brazil. HLA class I and II typing was performed at low/high resolution by using commercial kits, according to the manufacturer׳s protocol (One Lambda Inc., Canoga Park, CA). The allelic frequencies of the HLA class I and II Alisertib were estimated by direct counting. Comparison of allele frequency among the groups was performed by using Fisher׳s exact test or the Chi-square test. Significant P-values were corrected by the number of alleles tested for each locus. The relative risk (RR) 95% was estimated. Statistical analysis was performed with SAS 9.3 (SAS Institute Inc, EUA) and Epi InfoTM 7.0 (CDC, USA) software. Values P≤0.05 were considered significant. All the participants provided an informed written consent to participate in this study. The local Ethics Committee (#12248/2010) approved this study.
Acknowledgements This work was supported by FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) (#2010/51729-2). The first author received a post-doctorate scholarship from FAPESP, and the 2nd author received a doctorate scholarship from CNPq (Conselho Nacional de Pesquisa). The authors thank the clinical staff of the outpatient autoimmune dermatoses clinic at the University Hospital of Ribeirão Preto Medical School, University of São Paulo, Brazil.
1. Data Figs. 1–8 present the ENG of concentrating the DNA extract in a single amplification and the ENG of splitting the extract into two amplification tubes to produce two replicates. We call this the all in vs. two replicates data. Figs. 9–16 present the ENG of performing a DNA analysis to obtain a second replicate in a case where an electropherogram (EPG) has already been obtained from a first analysis. We call this the additional replicate data.
Experimental design, materials and methods
Disclaimer
Acknowledgements This work was supported by Grant number P2LAP3-148445 from the Swiss National Science Foundation. The authors sincerely thank John Buckleton, Steve Lund and one anonymous reviewer for their valuable comments.
Data The data provided here displays the synthesis, characterization, biocompatibility and hemocompatibility of siRNA-Npr3 loaded nanoparticles. Further, the gene silencing efficacy of the synthesized siRNA-Npr3 nanoparticles was demonstrated in the H9c2 cells in vitro and in rat hearts in vivo.